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The Control of pH with Synthetic Buffers

Thursday, July 5th, 2007

A series of hydrogen ion buffers covering the pKa range 6.15-8.60. Most of these buffers are amino acids. The synthetic buffer 4-2hydroxyethyl-1-piperazineethanesulfonic acid, HEPES, has gained widespread use recently.
The preservation of animal cells in liquid nitrogen, which may be seen under biology microscopes, is now a routine practice. Damage associated with freezing has been prevented […]

Osmolality in Culture Media

Thursday, July 5th, 2007

With the use of biology microscopes, it can be observed that living cells maintain an internal composition very different from, but highly dependent upon, their milieu. This is due to a combination of active and passive transport. In particular, cells respond by changes in size and shape to changes in the osmotic activity of […]

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